Regulatory

Part:BBa_K1484333

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI17, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1330
    Illegal EcoRI site found at 1401
    Illegal EcoRI site found at 1421
    Illegal PstI site found at 569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1330
    Illegal EcoRI site found at 1401
    Illegal EcoRI site found at 1421
    Illegal PstI site found at 569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1330
    Illegal EcoRI site found at 1401
    Illegal EcoRI site found at 1421
    Illegal BglII site found at 1574
    Illegal XhoI site found at 135
    Illegal XhoI site found at 215
    Illegal XhoI site found at 1209
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1330
    Illegal EcoRI site found at 1401
    Illegal EcoRI site found at 1421
    Illegal PstI site found at 569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1330
    Illegal EcoRI site found at 1401
    Illegal EcoRI site found at 1421
    Illegal PstI site found at 569
    Illegal NgoMIV site found at 1895
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1799


[edit]
Categories
//promoter
Parameters
None